Cell modeling of cardiomyocyte for studying the pathogenesis of the cardiovascular diseases

 

Most cells in adult organism are in differentiated state and unable or hardly able to differentiate into various directions, in vitro culturing and propagation. Nevertheless, most basic and applied research in medicine is carried out on cells in culture. This model allows us to study the electrophysiological characteristics, but not the development and functioning of cardiomyocytes. Most long-term studies on the culture of cardiomyocytes are conducted with using embryonic and neonatal cardiomyocytes, which excludes the possibility of such research on human beings.

Therefore, directed differentiation of various cell types in cardiomyocyte direction is the basic approach for providing a culture of human cardiomyocytes.

Opened at the beginning of the XXI century, resident heart multipotent stem cells is a promising source of cardiomyocyte culture. These cells can differentiate into cardiomyocytes , smooth muscle cells and endothelial cells. Undoubtedly, the resident stem cells are an attractive source for cardiomyocytes , but their limited proliferative potential and differentiation into cardiomyocytes in vitro is not effective enough. Therefore, our work is focused on obtaining functional human cardiomyocyte culture with using induced pluripotent stem cells (iPSCs).