Laboratory of Molecular and Cellular Mechanisms of Atherosclerosis

 
FedorovAV1Anton Fyodorov, PhD in Biology
Head of the Laboratory

The Laboratory focuses on vascular biology and studies the behavior patterns of endothelial and vascular smooth muscle cells, the mechanisms of their regulation and activation. Other important focus areas of the Laboratory are the regulatory properties of microRNA in relation to smooth muscle cells and the development of diagnostic approaches based on identification of cardiac specific microRNAs.

Research directions:

Participation in State Assignments

Since 2018, the Laboratory has been actively involved in the State Assignment «Circulating microparticles as endogenous carriers of nucleic acids in the pathogenesis of inflammatory and ischemic conditions of the cardiovascular system» (supervised by A. Fedorova).

Grants

  • MicroRNAs in response to ischemic myocardial injury: role in the regulation of myocardial cells and mechanisms of export to the circulatory system (Russian Foundation for Basic Research, Grant No. 14-04-01833, 2014–2016)
  • Studying the role of asymmetric dimethylarginine in the pathogenesis of myocardial dysfunction combined with ischemic injury and diabetes mellitus (Federal Targeted Programme No. 8489, 2012–2013)
  • Behavioral characteristics of muscle cells in vivo, in vitro and ex vivo in systems simulating the pathological development of cardiovascular system (State Contract No. 11.519.11.2018, 2011—2013)
  • Development of a new method for determining myocardial damage using microRNA study (State Contract No. 16.512.11.2043, 2011—2012)

Molecular mechanisms of restenosis and destabilization of atherosclerotic plaques (State Contract No. П1308, 2010—2012).

Major publications

Kondratov K., Fedorov A., Kostareva A., Golovkin A., Nikitin Y., Ivanov A., Mikhailovskii V., Isakov D. Heterogeneity of the nucleic acid repertoire of plasma extracellular vesicles demonstrated using high-sensitivity fluorescence-activated sorting. Journal of extracellular vesicles. 2020. Т. 9. № 1. С. 1743139. IF 14,976. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/32341769/.

Smolina N., Khudiakov A., Knyazeva A., Zlotina A., Sukhareva K., Kondratov K., Kostareva A., Sejersen T., Gogvadze V., Zhivotovsky B. Desmin mutations result in mitochondrial dysfunction regardless of their aggregation properties. Biochimica et Biophysica Acta (BBA)/Molecular Basis of Disease. 2020. Т. 1866. № 6. С. 165745. IF 4,352. Режим доступа: https://www.sciencedirect.com/science/article/pii/S0925443920300909.

Fedorov A.V., Kondratov K.A., Kostareva A.A., Golovkin A.S., Kishenko V., Vavilova T.V., Sirotkina O.V., Mikhailovskii V., Kudryavtsev I., Belyakova M., Sidorkevich S.V. Application of high-sensitivity flow cytometry in combination with low-voltage scanning electron microscopy for characterization of nanosized objects during platelet concentrate storage. Platelets. 2020. Т. 31. № 2. С. 226-235. IF 3,378. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/30977703/.

Tokarevich N.K., Panferova Y.A., Freylikhman O.A., Blinova O.V., Medvedev S.G., Mironov S.V., Grigoryeva L.A., Tretyakov K.A., Dimova T., Zaharieva M.M., Najdenski H., Nikolov B., Zehtindjiev P. Coxiella burnetii in ticks and wild birds. Ticks and Tick-borne Diseases. 2019. Т. 10. № 2. С. 377-385. IF 3,27. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/30509727/.

Baysa A., Valen G., Stensløkken K.O., Fedorov A.V., Kondratov K.A., Minasyan S.M., Galagudza M.M., Popov M., Kurapeev D.I., Yakovlev A., Kostareva A.A., Ruusalepp A., Vaage J. Release of mitochondrial and nuclear dna during on-pump heart surgery: kinetics and relation to extracellular vesicles. Journal of Cardiovascular Translational Research. 2019. Т. 12. № 3. С. 184-192. IF 2,48. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/30542983/.

Irtyuga O.B., Malashicheva A.B., Zhiduleva E.V., Freilikhman O.A., Rotar O.P., Back M., Tarnovskaya S.I., Kostareva A.A., Moiseeva O.M. Notch1 mutations in aortic stenosis: association with osteoprotegerin/rank/rankl. BioMed Research International. 2017. Т. 2017. С. 6917907. IF 2,276. Режим доступа:  https://pubmed.ncbi.nlm.nih.gov/28246602/.

Grigoryeva L.A., Samoylova E.P., Tokarevich N.K., Freilikhman O.A., Lunina G.A. Seasonal changes in populations of sheep tick, ixodes ricinus (l., 1758) (acari: ixodinae) in natural biotopes of st. Petersburg and leningrad province, russian federation. Systematic & Applied Acarology. 2019. Т. 24. № 4. С. 701-710. IF 1,76. Режим доступа: https://www.researchgate.net/publication/332765822_Seasonal_changes_in_populations_of_sheep_tick_Ixodes_ricinus_L_1758_Acari_Ixodinae_in_natural_biotopes_of_St_Petersburg_and_Leningrad_province_Russian_Federation.

Patents and inventions

Patent for invention RU 2683029 C1, 26.03.2019. Application No. 2018120414 dated 01.06.2018
“Method for detecting and assessing the DNA content of U. diversum by real-time PCR in adult cattle”
Authors: A. Vaganova, O. Freilikhman, S. Borisenko, V. Roka, V. Verbov

Patent for invention RU 2525059 C2, 10.08.2014. Application No. 2012130988/10 dated 19.07.2012
“Kit for detecting the causative agent of q-fever in biological material by real-time PCR method”
Authors: O. Freilikhman, N. Tokarevich, Yu. Panferova