Atherosclerosis Research Department

 

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Major research areas 

The Department focuses on the study of molecular factors associated with smooth muscle cells in the formation and destabilization of atherosclerotic plaques as well as in restenosis process after reconstructive surgery.

Structure

Participation in State Assignments

Since 2018, the Laboratory has been actively involved in the following state assignments:

  • Circulating microparticles as endogenous carriers of nucleic acids in the pathogenesis of inflammatory and ischemic conditions of the cardiovascular system (supervised by A. Fedorova)
  • Testing the effects of the combined use of periodic pneumatic compression and programmed electrical myostimulation for arterial blood flow disorders in the extremities (supervised by A. Krutikov)

Grants

  • MicroRNAs in response to ischemic myocardial injury: role in the regulation of myocardial cells and mechanisms of export to the circulatory system (Russian Foundation for Basic Research, Grant No. 14-04-01833, 2014–2016)
  • Studying the role of asymmetric dimethylarginine in the pathogenesis of myocardial dysfunction combined with ischemic injury and diabetes mellitus (Federal Targeted Programme No. 8489, 2012–2013)
  • Behavioral characteristics of muscle cells in vivo, in vitro and ex vivo in systems simulating the development of cardiovascular conditions (State Contract No. 11.519.11.2018, 2011—2013)
  • Development of a new method for determining myocardial damage using microRNA study (State Contract No. 16.512.11.2043, 2011—2012)
  • Molecular mechanisms of restenosis and destabilization of atherosclerotic plaques (State Contract No. П1308, 2010—2012)
  • Proteomic approach in the cultural property studies (Russian Science Foundation, Grant No. 19-73-00135, 2019—2021)
  • Analysis of big unstructured data for assessment system of recovery prognosis in integrative brain function and new treatments for impaired consciousness – a combination of loss and new pathological body integration” (Russian Foundation for Basic Research, Grant No. 19-29-01066, 2019—2021)
Major publications

Kondratov K., Fedorov A., Kostareva A., Golovkin A., Nikitin Y., Ivanov A., Mikhailovskii V., Isakov D. Heterogeneity of the nucleic acid repertoire of plasma extracellular vesicles demonstrated using high-sensitivity fluorescence-activated sorting. Journal of extracellular vesicles. 2020. Т. 9. № 1. С. 1743139. IF 14,976. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/32341769/.

Babakov V.N., Dubrovskii Y.A., Shreiner E.V., Keltsieva O.A., Sukhodolov N.G., Podolskaya E.P., Shilovskikh V.V., Zorin I.M., Zenkevich I.G., Selyutin A.A. Application of lanthanum stearate monolayers as a metal-affinity sorbent for the selective sorption of soman adducts to human serum albumin. Talanta. 2019. Т. 195. С. 728-731. IF 5,339. Режим доступа: https://www.sciencedirect.com/science/article/pii/S0039914018312554.

Dubrovskii Y., Solovyev N., Murashko E., Chuprina O., Beltyukov P., Radilov A., Babakov V. Mass spectrometry based proteomic approach for the screening of butyrylcholinesterase adduct formation with organophosphates. Talanta. 2019. Т. 197. С. 374-382. IF 5,339. Режим доступа: https://www.sciencedirect.com/science/article/pii/S0039914019300591.

Popova P.V., Vasileva L.B., Tkachuk A.S., Puzanov M.V., Bolotko Y.A., Pustozerov E.A., Gerasimov A.S., Zazerskaya I.E., Li O.A., Vasileva E.Yu., Kostareva A.A., Dmitrieva R.I., Grineva E.N. Association of tribbles homologue 1 gene expression in human umbilical vein endothelial cells with duration of intrauterine exposure to hyperglycaemia. Genetical Research. 2018. Т. 100. С. e3. IF 4,015. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/29502537/.

Smolina N., Khudiakov A., Knyazeva A., Zlotina A., Sukhareva K., Kondratov K., Kostareva A., Sejersen T., Gogvadze V., Zhivotovsky B. Desmin mutations result in mitochondrial dysfunction regardless of their aggregation properties. Biochimica et Biophysica Acta (BBA)/Molecular Basis of Disease. 2020. Т. 1866. № 6. С. 165745. IF 4,352. Режим доступа: https://www.sciencedirect.com/science/article/pii/S0925443920300909.

Fedorov A.V., Kondratov K.A., Kostareva A.A., Golovkin A.S., Kishenko V., Vavilova T.V., Sirotkina O.V., Mikhailovskii V., Kudryavtsev I., Belyakova M., Sidorkevich S.V. Application of high-sensitivity flow cytometry in combination with low-voltage scanning electron microscopy for characterization of nanosized objects during platelet concentrate storage. Platelets. 2020. Т. 31. № 2. С. 226-235. IF 3,378. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/30977703/.

Tokarevich N.K., Panferova Y.A., Freylikhman O.A., Blinova O.V., Medvedev S.G., Mironov S.V., Grigoryeva L.A., Tretyakov K.A., Dimova T., Zaharieva M.M., Najdenski H., Nikolov B., Zehtindjiev P. Coxiella burnetii in ticks and wild birds. Ticks and Tick-borne Diseases. 2019. Т. 10. № 2. С. 377-385. IF 3,27. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/30509727/.

Baysa A., Valen G., Stensløkken K.O., Fedorov A.V., Kondratov K.A., Minasyan S.M., Galagudza M.M., Popov M., Kurapeev D.I., Yakovlev A., Kostareva A.A., Ruusalepp A., Vaage J. Release of mitochondrial and nuclear dna during on-pump heart surgery: kinetics and relation to extracellular vesicles. Journal of Cardiovascular Translational Research. 2019. Т. 12. № 3. С. 184-192. IF 2,48. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/30542983/.

Irtyuga O.B., Malashicheva A.B., Zhiduleva E.V., Freilikhman O.A., Rotar O.P., Back M., Tarnovskaya S.I., Kostareva A.A., Moiseeva O.M. Notch1 mutations in aortic stenosis: association with osteoprotegerin/rank/rankl. BioMed Research International. 2017. Т. 2017. С. 6917907. IF 2,276. Режим доступа:  https://pubmed.ncbi.nlm.nih.gov/28246602/.

Grigoryeva L.A., Samoylova E.P., Tokarevich N.K., Freilikhman O.A., Lunina G.A. Seasonal changes in populations of sheep tick, ixodes ricinus (l., 1758) (acari: ixodinae) in natural biotopes of st. Petersburg and leningrad province, russian federation. Systematic & Applied Acarology. 2019. Т. 24. № 4. С. 701-710. IF 1,76. Режим доступа: https://www.researchgate.net/publication/332765822_Seasonal_changes_in_populations_of_sheep_tick_Ixodes_ricinus_L_1758_Acari_Ixodinae_in_natural_biotopes_of_St_Petersburg_and_Leningrad_province_Russian_Federation.

Patents and inventions

Patent for invention RU 2683029 C1, 26.03.2019. Application No. 2018120414 dated 01.06.2018
“Method for detecting and assessing the DNA content of U. diversum by real-time PCR in adult cattle”
Authors: A. Vaganova, O. Freilikhman, S. Borisenko, V. Roka, V. Verbov

Patent for invention RU 2525059 C2, 10.08.2014. Application No. 2012130988/10 dated 19.07.2012
“Kit for detecting the causative agent of q-fever in biological material by real-time PCR method”
Authors: O. Freilikhman, N. Tokarevich, Yu. Panferova