Gene and Cell Engineering Group

 

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Alexei Golovkin, MD, DSc
Head of the Group

The Group focuses on the development and testing of new tissue-engineered structures and modification of biomaterials in order to improve their biocompatibility and functionality. In particular, models of small-diameter vascular prostheses are being developed using surface-modified polymers and copolymers as well as decellularized umbilical arteries.

Another area is the study of the role of extracellular vesicles in the regulation of physiological and pathological processes in cells.

Projects

  1. Tissue-engineered biomaterials that stimulate the regenerative potential of tissues due to enhanced spatial architecture and reduced inflammatory response.
  2. Extracellular vesicles as biomarkers and regulators of cardiovascular conditions.

Grants

Own grants:

  • “Study of adenosine and TLR-mediated regulation in the processes of calcification of the elements of cardiovascular system using the model of aortic valve calcification” (RFBR grant No. 16-34-60199)
  • Study of the interaction of the pool of umbilical vein endothelial cells and human mesenchymal stem cells with biodegradable fibrous scaffolds based on polyesters with amino acids” (RFBR grant No. 17-33-50099)
  • “Molecular basis for the participation of extracellular vesicle subpopulations in the development of a systemic inflammatory response initiated by damage to the elements of cardiovascular system” (grant from the Russian Science Foundation No. 19-75-20076)

Contributors:

  • “Development and modeling of hybrid biodegradable scaffolds with predictable physicochemical and immunomodulatory properties for tissue-engineered structures” (grant from the Russian Science Foundation No. 16-13-10239, grant recipient: National Research Tomsk Polytechnic University)
  • Nanofiber 3D scaffolds based on polysaccharides for tissue engineering” (RFBR grant No. 18-29-17074, grant recipient: Institute of Macromolecular Compounds of the Russian Academy of Sciences)

Collaboration

  • National Research Tomsk Polytechnic University
  • Institute of Macromolecular Compounds of the Russian Academy of Sciences (Saint Petersburg)
  • Saint Petersburg State University
  • University of Cape Town, Cape Town, South Africa
Major publications

Kondratov K., Fedorov A., Kostareva A., Golovkin A., Nikitin Y., Ivanov A., Mikhailovskii V., Isakov D. Heterogeneity of the nucleic acid repertoire of plasma extracellular vesicles demonstrated using high-sensitivity fluorescence-activated sorting. Journal of extracellular vesicles. 2020. Т. 9. № 1. С. 1743139. IF 14,976. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/32341769/.

Bolbasov E.N., Khlusov I.A., Tverdokhlebov S.I., Popkov A.V., Popkov D.A., Gorbach E.N., Golovkin A.S., Sinev A., Bouznik V.M., Anissimov Y.G. Osteoinductive composite coatings for flexible intramedullary nails. Materials Science and Engineering: C. 2017. Т. 75. С. 207-220. IF 5,88. Режим доступа: https://www.sciencedirect.com/science/article/pii/S092849311730588X.

Kostina A., Shishkova A., Ignatieva E., Irtyuga O., Bogdanova M., Levchuk K., Golovkin A., Zhiduleva E., Uspenskiy V., Moiseeva O., Kostareva A., Malashicheva A., Faggian G., Vaage J., Rutkovskiy A. Different notch signaling in cells from calcified bicuspid and tricuspid aortic valves. Journal of Molecular and Cellular Cardiology. 2018. Т. 114. С. 211-219. IF 4,96. Режим доступа: https://www.sciencedirect.com/science/article/pii/S0022282817303449.

Toropova Ya., Golovkin A., Malashicheva A., Korolev D., Gorshkov A., Gareev K., Afonin M., Galagudza M. In vitro toxicity of FemOn, FemOn-SiO2 composite, and SiO2-FemOn core-shell magnetic nanoparticles. International Journal of Nanomedicine. 2017. Т. 12. С. 593-603. IF 4,471. Режим доступа: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245979/.

Semenova D., Bogdanova M., Kostina A., Golovkin A., Kostareva A., Malashicheva A. Dose-dependent mechanism of notch action in promoting osteogenic differentiation of mesenchymal stem cells. Cell and Tissue Research. 2019. № 10. С. 1007/s00441. IF 4,044. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/31781870/.

Semenova D., Bogdanova M., Kostina A., Golovkin A., Kostareva A., Malashicheva A. Dose-dependent mechanism of notch action in promoting osteogenic differentiation of mesenchymal stem cells. Cell and Tissue Research. 2020. Т. 379. № 1. С. 169-179. IF 4,044. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/31781870/.

Goreninskii S.I., Guliaev R.O., Stankevich K.S., Danilenko N.V., Filimonov V.D., Bolbasov E.N., Tverdokhlebov S.I., Golovkin A.S., Mishanin A.I. «Solvent/non-solvent» treatment as a method for non-covalent immobilization of gelatin on the surface of poly (l-lactic acid) electrospun scaffolds. Colloids and Surfaces B: Biointerfaces. 2019. Т. 177. С. 137-140. IF 4,04. Режим доступа: https://www.sciencedirect.com/science/article/pii/S0927776519300608.

Dmitrieva R.I., Khromova N.V., Golovkin A.S., Kostareva A.A., Lelyavina T.A., Galenko V.L., Bortsova M.A., Sitnikova M.Yu., Komarova M.Yu., Ivanova O.A., Sergushichev A., Tikanova P.A. Skeletal muscle resident progenitor cells coexpress mesenchymal and myogenic markers and are not affected by chronic heart failure-induced dysregulations. Stem Cells International. 2019. Т. 2019. С. 5690345. IF 3,902. Режим доступа: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335669/.

Kudryavtsev I.V., Serebriakova M., Isakov D., Zhiduleva E., Murtazalieva P., Titov V., Malashicheva A., Shishkova A., Semenova D., Irtyuga O., Moiseeva O., Golovkin A. CD73 rather than CD39 is mainly involved in controlling purinergic signaling in calcified aortic valve disease. Frontiers in genetics. 2019. Т. 10. № JUN. С. 604. IF 3,789. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/31402927/.

Knyazeva A., Krutikov A.N., Golovkin A.S., Mishanin A.I., Smolina N.A., Hushkina A., Galagudza M.M., Kostareva A.A., Pavlov G., Sejersen T., Sjoberg G. Time- and ventricular-specific expression profiles of genes encoding z-disk proteins in pressure overload model of left ventricular hypertrophy. Frontiers in genetics. 2019. Т. 9. № JAN. С. 684. IF 3,789. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/30666270/.

Kudryavtseva V., Stankevich K., Kibler E., Golovkin A.S., Mishanin A., Bolbasov E.N., Choynzonov E., Tverdokhlebov S.I. The deposition of thin titanium-nitrogen coatings on the surface of pcl-based scaffolds for vascular tissue engineering. Applied Physics Letters. 2018. Т. 112. № 15. С. 153705. IF 3,59. Режим доступа: https://tpu.pure.elsevier.com/ru/publications/the-deposition-of-thin-titanium-nitrogen-coatings-on-the-surface- .

Ignatieva E., Kostina D., Irtyuga O., Uspensky V., Golovkin A., Gavriliuk N., Moiseeva O., Kostareva A., Malashicheva A. Mechanisms of smooth muscle cell differentiation are distinctly altered in thoracic aortic aneurysms associated with bicuspid or tricuspid aortic valves. Frontiers in Physiology. 2017. Т. 8. № JUL. С. 536. IF 3,367. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/28790933/.

Goreninsky S.I., Bolbasov E.N., Sudarev E.A., Stankevich K.S., Anissimov Y.G., Golovkin A.S., Mishanin A.I., Viknianshchuk A.N., Filimonov V.D., Tverdokhlebov S.I. Fabrication and properties of L-arginine-doped PCL electrospun composite scaffolds. Materials Letters. 2018. Т. 214. С. 64-67. IF 3,204. Режим доступа: https://www.sciencedirect.com/science/article/pii/S0167577X17317408.

Bolbasov E.N., Khlusov I.A., Stankevich K.S., Ignatov V.P., Tverdokhlebov S.I., Popkov D.A., Kononovich N.A., Gorbach E.N., Popkov A.V., Golovkin A.S., Bouznik V.M., Anissimov Y.G. Flexible intramedullary nails for limb lengthening: a comprehensive comparative study of three nails types. Biomedical Materials (Bristol): Materials for tissue engineering and regenerative medicine. 2019. Т. 14. № 2. С. 025005. IF 3,174. Режим доступа: https://tpu.pure.elsevier.com/ru/publications/flexible-intramedullary-nails-for-limb-lengthening-a-comprehensiv.

Kozelskaya A.I., Bolbasov E.N., Golovkin A.S., Mishanin A.I., Viknianshchuk A.N., Shesterikov E.V., Ashrafov А., Novikov V.A., Fedotkin A.Y., Khlusov I.A., Tverdokhlebov S.I. Modification of the ceramic implant surfaces from zirconia by the magnetron sputtering of different calcium phosphate targets: a comparative study. Materials. 2018. Т. 11. № 10. С. 1949. IF 3,057. Режим доступа: https://pubmed.ncbi.nlm.nih.gov/30314394/.

Patents and inventions

Patent for invention RU 2564895 C1, 10.10.2015, application No. 2014123566/15 dated 09.06.2014.
«Method of manufacturing biological tissue samples in combination with implanted elements for imaging by light microscopy»
Authors: Mukhamadiyarov R., Sevostyanova V., Golovkin A., Nokhrin A.

Patent for invention RU 2504406 C1, 20.01.2014, application No. 2012149663/15 dated 21.11.2012.
«Method for manufacturing a bioresorbable hybrid vascular implant of small diameter»
Authors: Antonova L., Golovkin A., Barbarash O., Barbarash L.

Patent for invention RU 2520753 C1, 27.06.2014, application No. 2013105296/15 dated 07.02.2013.
«Method for predicting the development of a complicated systemic inflammatory response in patients with infective endocarditis»
Authors: Golovkin A., Ponasenko A., Grigoriev E., Shukevich D., Stasev A., Odarenko Yu., Barbarash L.

Patent for invention RU 2496526 C1, 27.10.2013, application No. 2012113439/15 dated 06.04.2012.
“Tissue-engineered vascular graft of small diameter and manufacturing method”
Authors: Barbarash L., Elgudin Ya., Sevostyanova V., Golovkin A.

Patent for invention RU 2463606 C1, 10.10.2012, application No. 2011134573/15 dated 17.08.2011.
«Method for early diagnosis of complicated systemic inflammatory response in patients undergoing surgery with cardiopulmonary bypass»
Authors: Golovkin A., Matveeva V., Grigoriev E., Shukevich D., Velikanova E., Barbarash L.