Head of the Laboratory, PhD
The main direction of the laboratory is the study of the functional state of different cell types in the development of pathological conditions such as obesity, diabetes, metabolic syndrome, heart failure.
One of the key objects of study are mesenchymal stromal cells: changing their proliferative and differentiation properties and changing regenerative potential in obesity and diabetes.
One of the research areas of the laboratory is studying the function of ion channel of cardiomyocytes under the influence of adipokines using Patch-Clamp technique. Then, another important methodological approach is the method of laser microdissection, that allows investigating the expression profile of individual cells and tissue structures separately.
- Stem cells as a model for studying the pathogenesis of cardiovascular system diseases
- Developing the cardiomyocyte cell model for studying the pathogenesis of the cardiovascular system diseases
- Molecular and genetic aspects of the metabolic syndrome development
Key publications of the Division
Dmitrieva R. I., Minullina I. R., Bilibina A. A., Tarasova O. V., Anisimov S. V., Zaritskey A. Y.
Bone marrow- and subcutaneous adipose tissue-derived mesenchymal stem cells: Differences and similarities. Cell Cycle. 2012 Jan 15; 11 (2): 377-83.
R. I. Dmitrieva, C. A. Hinojos, M. L. Grove, R. J. Bell, E. Boerwinkle, M. Fornage, P. A. Doris.
Genome-Wide Identification of Allelic Expression in Hypertensive Rats Circ Cardiovasc Genet. 2009; 2: 106-115.
Dmitrieva R. I., Hinojos C. A., Boerwinkle E., Braun M. C., Fornage M., Doris P.A.
Hepatocyte nuclear factor 1 and hypertensive nephropathy. Hypertension. 2008 Jun; 51 (6): 1583-9.
Tian D., Dmitrieva R. I., Doris P. A., Crary J. F., Sondhi R., Sacktor T. C., Bergold P. J.
Protein kinase M zeta regulation of Na/K ATPase: a persistent neuroprotective mechanism of ischemic preconditioning in hippocampal slice cultures. Brain Res. 2008 Jun 5; 1213: 127-39.
Karpushev A. V., Levchenko V., Ilatovskaya D. V., Pavlov T. S., Staruschenko A.
Novel role of Rac1/WAVE signaling mechanism in regulation of the epithelial Na+ channel. Hypertension. 2011; 57 (5): 996-1002.
Karpushev A. V., Vachugova D. V., Pavlov T. S., Negulyaev Y. A., Staruschenko A. Intact cytoskeleton is required for small G protein dependent activation of the epithelial Na+ channel. PLoS ONE. 2010; 5 (1): e8827.
Pochynyuk O., Kucher V., Boiko N., Mironova E., Staruschenko A., Karpushev A. V., Tong Q., Hendron E., Stockand J.
Intrinsic voltage dependence of the epithelial Na+ channel is masked by a conserved transmembrane domain tryptophan. J Biol Chem. 2009; 284 (38): 25512-21
Karpushev A. V., Ilatovskaya D. V., Staruschenko A.
The actin cytoskeleton and small protein RhoA are not involved in flow-dependent activation of ENaC. BMC Res Notes. 2010; 27 (3): 210.
Karpushev A. V., Levchenko V., Pavlov T. S., Lam V. Y., Vinnakota K. C., Vandewalle A., Wakatsuki T., Staruschenko A.
Regulation of ENaC expression at the cell surface by Rab11. Biochem Biophys Res Commun. 2008; 377 (2): 521-5.