Laboratory of Molecular Cardiology and Genetics

 

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Anna Malashicheva, PhD
Head of Laboratory

The main research area is the molecular basis of the pathogenesis of cardiovascular diseases. Major current projects focus on studying the cellular and molecular bases of the development of cardiomyopathy and ascending aortic diseases.

Also, the use of the approach of induced pluripotent human cells and release of C-kit + cardiac progenitor cells for the study of cell and molecular bases of cardiac and aortic diseases.

State Assignments

Since 2018, the specialists of the Research Laboratory have been participating in the following state assignments:

  • “Development of approaches for activation of the progenitor potential of resident cardiac stem cells for myocardial tissue regeneration”, supervised by A. Malashicheva.
  • “Study of the regulation mechanisms of the voltage-gated sodium channel Nav1.5 in order to find new targets for arrhythmia treatment” supervised by A. Karpushev.
  • “Study of genetic bases and development of new approaches to the diagnosis and treatment of isolated and combined congenital malformations” supervised by A. Kostareva.
  • “Circulating microparticles as endogenous carriers of nucleic acids in the pathogenesis of inflammatory and ischemic conditions of the cardiovascular system” supervised by A. Fedorov.
  • Development and implementation of new molecular genetic and proteomic approaches for the diagnosis of primary and secondary cardiomyopathies for selecting personalized therapy and for prognosis” supervised by A. Kostareva.

Projects

Ongoing research

Functional cardiomyocyte derived from human skin cells using pluripotent stem cell technology, stained for Troponin T

Endothelial aortic cells undergoing endothelial-to-mesenchymal transition and expressing smooth muscle actin

Human aortic endothelial cells, stained for CD31

Human aortic smooth muscle cells in aneurysm, stained for vimentin

Grants

  • Characteristics of the behavior of muscle cells in vivo, in vitro and ex vivo in systems simulating the development of cardiovascular conditions.
  • Role of the Notch1 gene in the development and functioning of the human aorta.
  • Genetic and pathophysiological study of the role of cytoskeletal proteins in the pathogenesis of cardiomyopathies
  • Use of induced pluripotent cell technology to study the pathogenesis of myopathies and cardiomyopathies associated with desmin gene mutations.
  • Molecular and cellular mechanisms underlying the development of laminopathies.
  • Study of the TGF-beta signaling pathway in smooth muscle cells in aortic aneurysm.

International projects

On 14 November 2019, the delegation from the University of Oslo headed by Dean of Medicine Ivar Prydz Gladhaug visited Almazov Centre. More

Major publications

Fishman V., Battulin N., Nuriddinov M., Korablev A., Serov O., Maslova A., Zlotina A., Chervyakova D., Krasikova A., Strunov A. 3D organization of chicken genome demonstrates evolutionary conservation of topologically associated domains and highlights unique architecture of erythrocytes' chromatin. Nucleic Acids Research. 2019. Т. 47. № 2. С. 648-665. IF 11,501. Access link: https://pubmed.ncbi.nlm.nih.gov/30418618/.

Smolina N., Bruton J., Sejersen T., Kostareva A. Sejersen. Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness. Methods in molecular biology (Clifton, N.J.). 2017. Т. 1601. С. 79-87. IF 10,710. Access link: https://pubmed.ncbi.nlm.nih.gov/28470519/.

Chiarini A., Marconi M., Armato U., Dal Pra I., Onorati F., Faggian G., Pasquali A., Patuzzo C., Pignatti P.F., Trabetti E., Malashicheva A., Irtyega O. Studies on sporadic non-syndromic thoracic aortic aneurysms: II. Alterations of extra-cellular matrix components and focal adhesion proteins. European Journal of Preventive Cardiology. 2018. Т. 25. № S1. С. 51-58. IF 5,864. Access link: https://pubmed.ncbi.nlm.nih.gov/29708036/.

Kudryavtseva V., Stankevich K., Bolbasov E., Zhuravlev M., Filimonov V., Remnev G., Tverdokhlebov S., Gudima A., Kibler E., Riabov V., Liu T., Klüter H., Kzhyshkowska J., Zhukov Y., Malashicheva A. Atmospheric pressure plasma assisted immobilization of hyaluronic acid on tissue engineering PLA-based scaffolds and its effect on primary human macrophages. Materials & design. 2017. Т. 127. С. 261-271.IF 5,770. Access link: https://www.sciencedirect.com/science/article/pii/S0264127517304367.

Kostina A., Shishkova A., Ignatieva E., Irtyuga O., Bogdanova M., Levchuk K., Golovkin A., Zhiduleva E., Uspenskiy V., Moiseeva O., Kostareva A., Malashicheva A., Faggian G., Vaage J., Rutkovskiy A. Different notch signaling in cells from calcified bicuspid and tricuspid aortic valves. Journal of Molecular and Cellular Cardiology. 2018. Т. 114. С. 211-219. IF 4,960. Access link: https://pubmed.ncbi.nlm.nih.gov/29158034/.

Rutkovskiy A., Bogdanova M., Stensløkken K. -O., Sullivan G., Fiane A., Vaage J., Malashicheva A., Kostareva A. Valve interstitial cells: the key to understanding the pathophysiology of heart valve calcification. Journal of the American Heart Association. 2017. Т. 6. № 9. С. e006339. IF 4,605. Access link: https://pubmed.ncbi.nlm.nih.gov/28912209/.

Lelyavina T.A., Galenko V.L., Ivanova O.A., Komarova M.Yu., Ignateva E.V., Bortsova M.A., Khromova N.V., Sitnikova M.Yu., Kostareva A.A., Dmitrieva R.I., Sergushichev A., Yukina G.Y. Clinical Response to Personalized Exercise Therapy in Heart Failure Patients with Reduced Ejection Fraction Is Accompanied by Skeletal Muscle Histological Alterations. International Journal of Molecular Sciences. 2019. Т. 20. № 21. С. 5514. IF 4,556. Access link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862491/.

Toropova Ya., Golovkin A., Malashicheva A., Korolev D., Gorshkov A., Gareev K., Afonin M., Galagudza M. In vitro toxicity of FemOn, FemOn-SiO2 composite, and SiO2-FemOn core-shell magnetic nanoparticles. IF 4,471. Access link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245979/.

Patents and inventions

Patent for invention RU 2689522 C1, 28.05.2019, application No. 2018132410 dated 11.09.2018.
«Nucleotide sequence encoding a fusion protein consisting of the soluble extracellular domain of human tnfr1 and the constant region of the heavy chain of human igg4»
Authors: Volkov K., Zavarzin A., Lukyanov D., Malashicheva A., Saifitdinova A., Semenikhin V., Shevchenko K., Lobov I.

Patent for invention RU 2554056 C1, 20.06.2015, application No. 2013140523/10 dated 03.09.2013.
«A set of synthetic oligonucleotides for determining the nucleotide sequence of the coding region of the nkx2.5, cfc1, gata4 genes and detecting mutations associated with orphan monogenic disease underlying familial forms of congenital heart defects»
Authors: Kostareva A., Zlotina A., Khodyuchenko T., Urusova M., Kiselev A.

Patent for invention RU 2556832 C2, 20.07.2015, application No. 2013127027/10 dated 06.06.2013.
«A set of synthetic oligonucleotides for determining the nucleotide sequence of the coding region of the des gene and detecting mutations associated with desmin cardiomyopathies»
Authors: Kostareva A., Smolina N., Malashicheva A., Urusova M., Kiselev A.

Patent for invention RU 2529947 C2, 10.10.2014, application No. 2012152110/10 dated 05.12.2012.
«Method for obtaining a culture of smooth muscle cells»
Authors: Malashicheva A., Kostareva A., Zverev D., Kostina D., Uspensky V., Gordeev M., Urusova M.