Research Laboratory of Operative Gynecology

 

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Комличенко Э.В. обработанное фотоIgor Govorov, MD, PhD (Karolinska Institutet)
Head of Laboratory, Ob/Gyn

The major task of the Laboratory is to study the characteristics of the pathogenesis and clinical course of the most common gynecological diseases.

The Laboratory closely cooperates with medical departments of the Perinatal Centre, Children's Rehabilitation Clinic, Preclinical Translational Research Centre.

The Laboratory deals with a wide range of research tasks using methods of molecular biology, omics technologies, animal experiments, modern IT solutions.

Major research areas (2017—2019)

  • Robot-assisted oophorectomy and subsequent cryopreservation of ovarian tissue to preserve fertility in girls with cancer
  • Innovative approaches to the diagnosis and treatment of noncommunicable diseases of the female genital organs based on the methods of metabolomics and metagenomics.
  • Development and training of a neural network for analysis of medical images, primarily colposcopic ones

Collaboration, joint projects and grants

  • Karolinska Institute (Stockholm, Sweden)
    Project dedicated to the most common hereditary blood-clotting disorder Von Willebrand disease.
  • University of Perugia (Perugia, Italy)
    Possibilities of 3D-printing in perinatology and pediatrics.
  • Petersburg University of Information Technologies, Mechanics and Optics (St. Petersburg, Russia)
    Project aimed at studying potential biomarkers of clinically most significant gynecologic conditions, primarily endometriosis (using omics technologies and mass spectrometry).
  • Peter the Great St. Petersburg Polytechnic University (St. Petersburg, Russia)
    The current project is dedicated to computer analysis of medical images using machine learning methods: computer analysis of endoscopic mucosal images for the early diagnosis of gynecological cancers. A neural network model capable of differentiating images obtained by colposcopy was developed.
  • European University (St. Petersburg, Russia)
    Patient-centered approach in Russian health care: organizational challenges and opportunities for professionals.

Results of research

Разработан метод дериватизации нескольких классов соединений для их определения в образце биологического происхождения с помощью изобутилхлорформиата.

Metabolome project:

•Developed a derivatization method for several classes of compounds for their determination in a biological sample with isobutyl chloroformate.
•Validated the parameters of biological sample preparation for the determination of several classes of compounds, identified the parameters of water extraction and its advantages over other types of extraction.
•Identified the substances detected by the analysis of patient biological samples, determined the content ratio of 3 classes of specific metabolites in the affected tissues – mono- and dicarbonic acids, amino acids.
•The analytical reliability for selected metabolites was more than 85% with Lab Solutions (Shimadzu) software.

Oncofertility project:

We have developed a method for vitrification of ovarian tissue followed by orthotopic autotransplantation in an animal experiment. The results were summarized in an article published in a foreign peer-reviewed journal. The method is supposed to be implemented in clinical practice, primarily in patients receiving gonadotoxic treatment.

Major publications

Razumova Z., Oda H., Govorov I., Lundin E., Östensson E., Lindquist D., Mints M… The Prognostic Role of LRIG Proteins in Endometrial Cancer. Cancers. 2021; 13 (6): 1361 1-13. IF 6,102. Access link: https://doi.org/10.3390/cancers13061361

Razumova Z., Govorov I., Östensson E., Mints M. Cadmium Intake as a Prognostic Factor in Endometrial Cancer: A Swedish Cohort-Based Study. Nutrition and Cancer. 2021; 1-9 . IF 2,409. Access link: https://doi.org/10.1080/01635581.2021.1883681

Igor Govorov, Elena Dikareva,  Elena Velichko,  Vesna Kesic,  Eduard Komlichenko. Human-machine alliance against cervical cancer. 2021; 31 (2): 299-299. IF 2,117. Access link: https://doi.org/10.1136/ijgc-2020-002064

Averkin, II, Grehov E. V., Pervunina T. M., Komlichenko E. V., Vasichkina E. S., Zaverza V. M., Nikiforov V. G., Latipova M. L., Govorov I. E., Kozyrev I. A., Komlichenko E. E., Ricci A., Biccari E., Tsibizova V. I. 3D-printing in preoperative planning in neonates with complex congenital heart defects // J Matern Fetal Neonatal Med. ‒ 2020.10.1080/14767058.2020.1771691. ‒ C. 1-5.

Tsibizova V. I., Pervunina T. М., Komlichenko E. V., Govorov I. E., Averkin I. I., Makatsariya A. D., Di Renzo G. C. Cardiac function in TTTS twins after laser coagulation // Prenatal Cardiology. ‒ 2020. ‒ T. 2020, № 1. ‒ C. 1-4.

Makatsariya A. D., Slukhanchuk E. V., Bitsadze V. O., Khizroeva J. K., Tretyakova M. V., Tsibizova V. I., Shkoda A. S., Grandone E., Elalamy I., Rizzo G. COVID-19, hemostasis disorders and risk of thrombotic complications // Annals of the Russian academy of medical sciences. ‒ 2020. ‒ T. 75, № 4. ‒ C. 306-317.

Makatsariya A., Slukhanchuk E., Bitsadze V., Khizroeva J., Tretyakova M., Tsibizova V., Dobryakov A., Elalamy I., Gris J. C. COVID-19, neutrophil extracellular traps and vascular complications in obstetric practice // J Perinat Med. ‒ 2020. ‒ T. 48, № 9. ‒ C. 985-994.

Sharma D., Tsibizova V. I. Current perspective and scope of fetal therapy: part 1 // The Journal of Maternal-Fetal & Neonatal Medicine. ‒ 2020. ‒ C. 1-29.

Neykova K., Tosto V., Giardina I., Tsibizova V., Vakrilov G. Endometrial receptivity and pregnancy outcome // J Matern Fetal Neonatal Med. ‒ 2020.10.1080/14767058.2020.1787977. ‒ C. 1-15.

Tsibizova V., Govorov I., Pervunina T., Komlichenko E., Kudryashova E., Blinov D., Makatsariya A., Di Renzo G. First trimester prenatal screening in multiple pregnancies. Part I: comparative analysis of serum proteins PAPP-A and p-hCG in pregnancies conceived spontaneously or by in vitro fertilization // Obstetrics, gynecology and reproduction. ‒ 2020. ‒ T. 14, № 1. ‒ C. 25-33.

Tsibizova V., Govorov I., Pervunina T., Komlichenko E., Kudryashova E., Blinov D., Makatsariya A., Di Renzo G. First trimester prenatal screening in multiple pregnancies. Part II: serum proteins PAPP-A and β-hCG as markers of adverse pregnancy outcomes // Obstetrics, gynecology and reproduction. ‒ 2020. ‒ T. 14, № 1. ‒ C. 34-43.

Tsibizova V., Govorov I., Averkin I., Khamani N., Blinov D. Health technology assessment in obstetrics: advantage of tailored conservative strategy vs surgical therapies of monochorionic twin complicated by TRAP-sequence // FARMAKOEKONOMIKA. Modern Pharmacoeconomic and Pharmacoepidemiology. ‒ 2020. ‒ T. 13, № 1. ‒ C. 36-42.

Govorov I., Dikareva E., Velichko E., Kesic V., Komlichenko E. Human-machine alliance against cervical cancer // Int J Gynecol Cancer. ‒ 2020.10.1136/ijgc-2020-002064. ‒ C. ijgc-2020-002064.

Khizroeva J. K., Makatsariya A. D., Bitsadze V. O., Tretyakova M. V., Slukhanchuk E. V., Elalamy I., Gris J. C., Radetskaya L. S., Makatsariya N. A., Sulina Y. Y., Tsibizova V.I. Laboratory monitoring of COVID-19 patients and importance of coagulopathy markers // Obstetrics, Gynecology and Reproduction. ‒ 2020. ‒ T. 14, № 2.

Govorov I., Sitkin S., Pervunina T., Moskvin A., Baranenko D., Komlichenko E. Metabolomic Biomarkers in Gynecology: A Treasure Path or a False Path? // Curr Med Chem. ‒ 2020. ‒ T. 27, № 22. ‒ C. 3611—3622.

Bitsadze V. O., Grigoreva K., Khizroeva J. K., Pervunina T. M., Tsibizova V. I., Tretyakova M. V., Makatsariya A. D. Novel coronavirus infection and Kawasaki disease // J Matern Fetal Neonatal Med. ‒ 2020.10.1080/14767058.2020.1800633. ‒ C. 1-5.

Di Renzo G. C., Makatsariya A. D., Tsibizova V. I., Capanna F., Rasero B., Komlichenko E. V., Pervunina T. M., Khizroeva J. K., Bitsadze V. O., Shkoda A. S. Obstetric and perinatal care units functioning during the COVID-19 pandemic // Annals of the Russian academy of medical sciences. ‒ 2020. ‒ T. 75, № 1. ‒ C. 83-92.

Startseva T. V., Kanshina N. N., Tretyakova M. V., Bitsadze V. O., Khizroeva J. K., Radetskaya L. S., Stuleva N. S., Tsibizova V. I., Shkoda A. S., Blinov D. V. Pregnancy and Zika virus // Obstetrics, Gynecology and Reproduction. ‒ 2020. ‒ T. 14, № 2. ‒ C. 229-238.

Capanna F., Haydar A., McCarey C., Bernini Carri E., Bartha Rasero J., Tsibizova V., Helmer H., Makatsarya A., Di Renzo G. C. Preparing an obstetric unit in the heart of the epidemic strike of COVID-19: quick reorganization tips // J Matern Fetal Neonatal Med. ‒ 2020.10.1080/14767058.2020.1749258. ‒ C. 1-7.

Tosto V., Giardina I., Tsibizova V., Di Renzo G. C. Preterm Birth, From the Biological Knowledges to the Prevention: An Overview // Maternal-Fetal Medicine. ‒ 2020. ‒ T. 2, № 3. ‒ C. 162-171.

Di Renzo G. C., Tosto V., Tsibizova V. Progesterone: History, facts, and artifacts // Best Pract Res Clin Obstet Gynaecol. ‒ 2020. ‒ T. 69. ‒ C. 2-12.

Makatsariya A. D., Di Renzo G. C., Rizzo G., Bitsadze V. O., Khizroeva J. K., Blinov D. V., Vovk E. I., Govorov I. E., Guryev D. L., Dikke G. B., Tsibizova V.I. Regarding the evidence-based use of micronized progesterone // Obstetrics, Gynecology and Reproduction. ‒ 2020. ‒ T. 14, № 3. ‒ C. 374-383.

Govorov I. E., Vorobeva M. A., Komlichenko E. V. Robotic repair of the symptomatic vesicouterine fistula // Int Urogynecol J. ‒ 2020.10.1007/s00192-020-04558-0. ‒ C. 1-3.

Bitsadze V. O., Sukontseva T. A., Akinshina S. V., Sulina Y. Y., Khizroeva J. K., Tretyakova M. V., Sultangadzhieva K. G., Ungiadze J. Y., Samburova N. V., Grigoreva K. N., Tsibizova V.I. Septic shock // Obstetrics, Gynecology and Reproduction. ‒ 2020. ‒ T. 14, № 3. ‒ C. 314-326.

Makatsariya A. D., Slukhanchuk E. V., Bitsadze V. O., Khizroeva J. K. H., Tretyakova M. V., Tsibizova V. I., Elalamy I., Gris J. C., Grandone E., Makatsariya N. A., Mashkova T. Thrombotic microangiopathy, DIC-syndrome and COVID-19: link with pregnancy prothrombotic state // J Matern Fetal Neonatal Med. ‒ 2020.10.1080/14767058.2020.1786811. ‒ C. 1-9.

Metabolomic biomarkers in gynecology: a treasure path or a false path? I. Govorov, S. Sitkin, T. Pervunina [et al.] // Curr Med Chem. 2019. DOI:10.2174/0929867326666190104124245

Blood inflammatory and endothelial markers in women with von Willebrand disease / I. Govorov, K. Bremme, A. Larsson [et al.] // PLoS One. 2019. V. 14 (1). e0210544. DOI:10.1371/journal.pone.0210544

Ovarian tissue cryopreservation in prepubertal patients with oncological diseases: multidisciplinary approach and outcomes / Y. V. Dinikina, M. B. Belogurova, A. Y. Zaritskey [et al.] // The Journal of Maternal-Fetal & Neonatal Medicine. 2019. P. 1–231.

On specific features of the endoscopic image processing M. Galeeva, M. Baranov, V. Pavlov [et al.] // Journal of Physics: Conference Series. T. 1236. IOP Publishing, 2019. C. 012036.

A concept of smart medical autonomous distributed system for diagnostics based on machine learning technology / E. Velichko, E. Nepomnyashchaya, M. Baranov [et al.] // Internet of Things, Smart Spaces, and Next Generation Networks and Systems Springer, Cham, 2019. P. 515–

Thrombin generation during a regular menstrual cycle in women with von Willebrand disease / I. Govorov, K. Bremme, T. L. Lindahl [et al.] // Scientific reports. 2018. 8 (1), 17467.

Govorov I. Von Willebrand disease in women: heavy menstrual bleeding and obstetrical bleeding, 2018.

Advanced techniques in biology & medicine the fluctuations in homocysteine level caused by various combinations of folic acid cycle genes snp alleles as a factor in the course of pregnancy violation / E. V. Komlichenko, Y. N. Fedotov, M. A. Uvarova // Advanced Techniques in Biology & Medicine. 2018. 6 (1), P. 1–6. DOI: 10.4172/2379—1764.1000254.