Research Laboratory of Operative Gynecology

 
Комличенко Э.В. обработанное фотоEduard Komlichenko, MD, DSc
Head of Laboratory, Deputy Director for Science and Clinic of the Institute of Perinatology and Pediatrics, Ob/Gyn

The major task of the Laboratory is to study the characteristics of the pathogenesis and clinical course of the most common gynecological diseases. A separate area of research involves the development and optimization of fertility preservation methods in patients undergoing gonadotoxic treatment.

The Laboratory closely cooperates with medical departments of the Perinatal Centre, Children's Rehabilitation Clinic, Preclinical Translational Research Centre.

Major research areas (2017—2019)

  • Innovative approaches to the diagnosis and treatment of noncommunicable diseases of the female genital organs based on the methods of metabolomics and metagenomics.
  • Robot-assisted oophorectomy and subsequent cryopreservation of ovarian tissue in order to maintain fertility in girls with cancer.
  • Development and optimization of the method for cryopreservation of ovarian tissue in order to maintain reproductive health in cancer patients.
  • Development and optimization of organ-sparing robotic surgery for ovarian masses.

Collaboration, joint projects

  • Karolinska Institute (Stockholm, Sweden)
    Project dedicated to the most common hereditary blood-clotting disorder Von Willebrand disease.
  • University of Perugia (Perugia, Italy)
    Possibilities of the 3D-printing method in perinatology and pediatrics.
  • Petersburg National Research University of Information Technologies, Mechanics and Optics (St. Petersburg, Russia)
    Project aimed at studying potential biomarkers of clinically most significant gynecologic conditions, primarily endometriosis (using ohmic technology and mass spectrometry).
  • Peter the Great St. Petersburg Polytechnic University (St. Petersburg, Russia)
    The current project is dedicated to computer analysis of medical images using machine learning methods: computer analysis of endoscopic mucosal images for the early diagnosis of gynecological cancers. A neural network model capable of differentiating images obtained by colposcopy was developed. Neural network training is in progress.
  • European University (St. Petersburg, Russia)
    Patient focus in Russian health care: organizational challenges and opportunities for professionals.

Results of research

Metabolome project:

  • Developed a derivatization method for several classes of compounds for their determination in a biological sample with isobutyl chloroformate.
  • Validated the parameters of biological sample preparation for the determination of several classes of compounds, identified the parameters of water extraction and its advantages over other types of extraction.
  • Identified the substances detected by the analysis of patient biological samples, determined the content ratio of 3 classes of specific metabolites in the affected tissues – mono- and dicarbonic acids, amino acids.
  • The analytical reliability for selected metabolites was more than 85% with Lab Solutions (Shimadzu) software.

Oncofertility project:

We have developed a method for vitrification of ovarian tissue followed by orthotopic autotransplantation in an animal experiment. The results were summarized in an article published in a foreign peer-reviewed journal. The method is supposed to be implemented in clinical practice, primarily in patients receiving gonadotoxic treatment.

Educational and publishing highlights

  • Defense of PhD thesis: Igor Govorov, Karolinska Institute, Sweden (2014–2018).
  • Scientific advice and editing: Atlas of Pelvic Anatomy and Gynecologic Surgery 4th Edition by Michael S. Baggish and Mickey M. Karram, 2018.
  • Scientific advice and editing: Practical Obstetrics, Joachim W. Dudenhausen, 21th edition, 2019.
  • Contribution to the issue of the Journal, Obstetrics and Gynecology of St. Petersburg, specialists of Operative Gynecology Laboratory (journal website).

Participation in conferences (2017—2019)

  • Scientific and practical conference, Topical Issues of Perinatal Obstetrics (February, 2017)
  • Scientific and practical conference, Perinatal Medicine From its Origin to the Current State (October, 2017)
  • Interregional scientific and practical conference, Topical Issues of Obstetrics and Gynecology 2017 (December, 2017)
  • All-Russian conference with international participation, Hemostasis, Thrombosis and Reproduction: an Interdisciplinary Approach (March, 2018)
  • Scientific and educational seminar, Atypical Hemolytic-Uremic Syndrome: a Complex Diagnosis in the Practice of Doctors of Different Specialties (October, 2018)
  • High-Tech Medicine in Gynecology: the case study of the Institute of Perinatology and Pediatrics (march, 2019)

International collaboration

  • Innovative developments in gynecological laparoscopy: Botchorishvili R. (Clermont-Ferrand, France)
  • Use of CO2 laser: Donnez J., Donnez O. (Universite Catholique de Louvain, Belgium)
  • Oncofertility: Donnez J., Dolmans M-M. (Universite Catholique de Louvain, Belgium)
  • da Vinci robotic system (University of Rome La Sapienza)
  • Neurogenesis: Donnez O. (Universite Catholique de Louvain, Belgium)
  • Surgical navigation systems: Breimer K. Zürcher (Fachhochschule, Germany)
  • Metabolomics
Major publications

Metabolomic biomarkers in gynecology: a treasure path or a false path? I. Govorov, S. Sitkin, T. Pervunina [et al.] // Curr Med Chem. 2019. DOI:10.2174/0929867326666190104124245

Blood inflammatory and endothelial markers in women with von Willebrand disease / I. Govorov, K. Bremme, A. Larsson [et al.] // PLoS One. 2019. V. 14 (1). e0210544. DOI:10.1371/journal.pone.0210544

Ovarian tissue cryopreservation in prepubertal patients with oncological diseases: multidisciplinary approach and outcomes / Y. V. Dinikina, M. B. Belogurova, A. Y. Zaritskey [et al.] // The Journal of Maternal-Fetal & Neonatal Medicine. 2019. P. 1–231.

On specific features of the endoscopic image processing M. Galeeva, M. Baranov, V. Pavlov [et al.] // Journal of Physics: Conference Series. T. 1236. IOP Publishing, 2019. C. 012036.

A concept of smart medical autonomous distributed system for diagnostics based on machine learning technology / E. Velichko, E. Nepomnyashchaya, M. Baranov [et al.] // Internet of Things, Smart Spaces, and Next Generation Networks and Systems Springer, Cham, 2019. P. 515–

Thrombin generation during a regular menstrual cycle in women with von Willebrand disease / I. Govorov, K. Bremme, T. L. Lindahl [et al.] // Scientific reports. 2018. 8 (1), 17467.

Govorov I. Von Willebrand disease in women: heavy menstrual bleeding and obstetrical bleeding, 2018.

Advanced techniques in biology & medicine the fluctuations in homocysteine level caused by various combinations of folic acid cycle genes snp alleles as a factor in the course of pregnancy violation / E. V. Komlichenko, Y. N. Fedotov, M. A. Uvarova // Advanced Techniques in Biology & Medicine. 2018. 6 (1), P. 1–6. DOI: 10.4172/2379—1764.1000254.